A simple and feasible approach to purify konjac glucomannan from konjac flour--temperature effect.

نویسندگان

  • Wei Xu
  • Sujuan Wang
  • Ting Ye
  • Weiping Jin
  • Jinjin Liu
  • Jieqiong Lei
  • Bin Li
  • Chao Wang
چکیده

A simple one-step purification process was provided to extract KGM from KF by phase separation. The results showed that appropriate temperature control was a key factor and the products were inodorous, colourless and of high purity at the optimal temperature 68 °C. In this purification, soluble sugar and starch of extracted KGM were nearly clearly reduced and up to 95%, 80% (T68) of protein and ash were removed, respectively as compared with KF. Odour and transparency were improved 4 ranks and 30%, respectively. Besides, the ηapp reached 42.30 Pa s and increased by 93.55% as compared, which could stay at a steady level for a week. Furthermore, morphology of extracted KGM displayed regular lamellar and wrinkling distribution for removed impurities. The temperature-controlled method not only enriches the knowledge of KGM purification but also has the potential to broaden the application of KGM.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Laxative Potential of The Konjac Flour (Amorphophallus muelleri Blume) in Treatment of Loperamide Induced Constipation on Sprague Dawley Rats

There is long history of konjac tubers being used as a cure for certain diseases in China and Japan. Konjac flour is prepared from konjac tubers and it contains high concentration of glucomannan. Konjac Glucomannan (KGM) is dietary fiber and the role of which has been demonstrated in weight reduction, lowering blood cholesterol and sugar level, promoting intestinal activity etc. Konjac glucoman...

متن کامل

Rheological Properties of Mixtures of Spruce Galactoglucomannans and Konjac Glucomannan or Some Other Polysaccharides

Spruce galactoglucomannan is a wood-derived polysaccharide with a modest molar mass that has recently been made available in kg-scale for research and development of value-added products. To promote the application of spruce galactoglucomannans in, for example, food products, it is vital to understand also the rheological behaviour of the mixtures of galactoglucomannans with other polysaccharid...

متن کامل

Viscosity and Gel Formation of a Konjac Flour from Amorphophallus oncophyllus

The influences of different variables on the viscosity and gel formation of the konjac flour produced from Amorphophallus oncophyllus have been studied. At the concentration studied, the 1.5% konjac flour yielded a viscous solution than that of 0.5% konjac flour. The viscosity of konjac flour solution were unaffected by pH (pH 2 to pH 8), salt concentration (0-10% w/v), and high acid (pH 3.5) /...

متن کامل

De Novo Transcriptome and Small RNA Analyses of Two Amorphophallus Species

Konjac is one of the most important glucomannan crops worldwide. The breeding and genomic researches are largely limited by the genetic basis of Amorphophallus. In this study, the transcriptomes of A. konjac and A. bulbifer were constructed using a high-throughput Illumina sequencing platform. All 108,651 unigenes with average lengths of 430 nt in A. konjac and 119,678 unigenes with average len...

متن کامل

Deciphering Ligand Specificity of a Clostridium thermocellum Family 35 Carbohydrate Binding Module (CtCBM35) for Gluco- and Galacto- Substituted Mannans and Its Calcium Induced Stability

This study investigated the role of CBM35 from Clostridium thermocellum (CtCBM35) in polysaccharide recognition. CtCBM35 was cloned into pET28a (+) vector with an engineered His6 tag and expressed in Escherichia coli BL21 (DE3) cells. A homogenous 15 kDa protein was purified by immobilized metal ion chromatography (IMAC). Ligand binding analysis of CtCBM35 was carried out by affinity electropho...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Food chemistry

دوره 158  شماره 

صفحات  -

تاریخ انتشار 2014